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KMID : 0368019940170041395
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Journal of Soonchunhyang University
1994 Volume.17 No. 4 p.1395 ~ p.1410
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The Infection of Salmonella species in Human Vascular Endothelial Cells in Vitro
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Abstract
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In order to elucidate the mechanism of injury to endothelial cell of blood vessels in typhoid fever, Salmonella typhi was inoculated in cultured human endothelial cells . Endothelial cells were prepared from the umbilical vein that was freshly
obtained
form human umbilical cord by collagenase treatment. After screening of cultured endothelial cells through the immunofluorescent antibody technique, ultrastructural change of cytopathic Aeffect, obtained by lectron microscopy, showed typical
progress of
pathogenesis caused by S. typhi; adherence, endocytosis, intracellular replication and release of microorganism. These patholgical changes led to cellular disruption and final death of endothelial cells.
Endothelial cells of vascular system found to be the site of amplification as well as the target organ of S.typhi, which could play important role in hematogenous spread of this microorganism and also in pathologic sign or symptom in typhoid
fever.
An experimental infection of S. typhi in cultured human endothelial cells found to be an excellent and valuable virulence assay system. The above ability of S. typhi to proliferate and lead to endothelial cell death in cultured human endothelial
cells
confirmed to be a factor of endovascular disorder of typhoid fever such as rose spot on abdomen of patients.
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